Erik Kish-Trier (Presenter)
ARUP Laboratories
Authorship: Erik Kish-Trier, Aiping Liu, Maria Erali, Tatiana Yuzyuk
ARUP Institute for Clinical and Experimental Pathology,
| Short Abstract Long chain fatty acids (LCFAs) play diverse and critical biological roles. Here we present a GC-NCI-MS method for quantitation of 22 LCFAs (C12-C22) in serum/plasma, including the essential and conditionally essential polyunsaturated LCFAs. Hydrolysis was used to release bound LCFAs, which were extracted in hexane and derivatized to form pentafluorobenzyl esters. Our GC strategy gave baseline separation for most analytes, prior to being ionized by NCI and detected in SIM mode. This method offers several advantages upon published studies by using a single injection, additional internal standards, and backflush technology. Validation data and updated reference ranges are presented. |
Long Abstract
Long chain fatty acids (LCFAs) play diverse and critical biological roles. Here we present a GC-NCI-MS method for quantitation of 22 LCFAs (C12-C22) in serum/plasma, including the essential and conditionally essential polyunsaturated LCFAs. Hydrolysis was used to release bound LCFAs, which were extracted in hexane and derivatized to form pentafluorobenzyl esters. Our GC strategy gave baseline separation for most analytes, prior to being ionized by NCI and detected in SIM mode. This method offers several advantages upon published studies by using a single injection, additional internal standards, and backflush technology. Validation data and updated reference ranges are presented.
References & Acknowledgements:
| Description | Y/N | Source |
| Grants | no | |
| Salary | yes | ARUP Laboratories |
| Board Member | no | |
| Stock | no | |
| Expenses | yes | ARUP Laboratories |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | no |