Marta Kozak (Presenter)
ThermoFisher Scientific
Authorship: Nathaly Reyes Garces1, German Augusto Gomez-Rios1, Cornelia Boeser2, David Sarracino2, Marta Kozak2, Janusz Pawliszyn1
1Waterloo University, 2Thermo Fisher Scientific
| Short Abstract The novel sample preparation method using solid phase micro extraction (SPME) blades was evaluated for analysis of 20 chemically diverse drugs of abuse in urine and plasma samples. Analytes were detected on a hybrid quadrupole-Orbitrap-based mass spectrometer. Urine and plasma samples were spiked with internal standards then diluted with buffer and water, respectively. The sample preparation method consisted of 4 steps which included: SPME blades conditioning, analytes extraction, wash step and elution step. Method was evaluated for limits of quantitation, precision and matrix effects. Limits of quantitation were in range of 0.1-5 ng/mL and method precision was better than 15% in both urine and plasma. Matrix effects in urine samples were compounds dependent and were in the range of 27.1-100 % recovery. Negligible matrix effects in plasma were observed: 79.0-119 % recovery. |
Long Abstract
The novel sample preparation method using solid phase micro extraction (SPME) blades was evaluated for analysis of 20 chemically diverse drugs of abuse in urine and plasma samples. Analytes were detected on a hybrid quadrupole-Orbitrap-based mass spectrometer which collected full scan data with resolution of 60K. Urine and plasma samples were spiked with internal standards then diluted with buffer and water, respectively. The sample preparation method consisted of 4 steps which included: SPME blades conditioning, analytes extraction, wash step and elution step. The method was implemented on an automated autosampler which processed the samples in a 96-well plate. Method was evaluated for limits of quantitation, precision and matrix effects. Data were processed by reconstructing analyte chromatographic peaks with mass accuracy of 5 ppm.
Limits of quantitation were in range of 0.1-5 ng/mL, the upper calibration range for all analytes was 100 ng/mL (the highest concentration evaluated) and method precision was better than 15% in both urine and plasma. Matrix effects in urine samples were compounds dependent and were in the range of 27.1-100 % recovery. Negligible matrix effects in plasma were observed: 79.0-119 % recovery.
References & Acknowledgements:
| Description | Y/N | Source |
| Grants | no | |
| Salary | yes | ThermoFisher Scientific |
| Board Member | no | |
| Stock | no | |
| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | no |