Edward Faden (Presenter)
MAC-MOD Analytical
Bio: Edward Faden received his Bachelor's Degree in Chemistry from University of Pittsburgh in 2013. He worked in a drug discovery laboratory under the guidance of Billy Day. Edward is the Regional Sales Manager and Bioclass Product Manager for MAC-MOD Analytical. In this role, Edward builds marketing, product position, and sales strategies for the HALO BioClass Fused-Core Column product line. Edward has been working with MAC-MOD Analytical since 2013, when he joined MAC-MOD as a Chromatography Specialist intern. He was exceptional in his role and since then has been added to the MAC-MOD team in late 2013.
Authorship: Benjamin libert (1) William Miles (1) Stephanie Schuster (1) Barry Boyes (1) Edward Faden (2) Thomas Waeghe (2)
(1) Advanced Materials Technology (2) MAC-MOD Analytical
| Short Abstract The structural characterization of monoclonal antibodies (mAbs) is a challenging and complex task. Efforts have been directed at optimizing high performance HALO® BioClass Fused-Core™ silica materials in order to improve the separations component of LC-MS analysis of proteins, mAbs, glycopeptides and released glycans. Recently improved superficially porous silica packing materials have been applied to HPLC / UHPLC characterization of biomolecules including glycosylation variants from mAbs. HALO BioClass Fused-Core™ materials show considerable utility for analysis of these highly complex molecules, using conditions that permit excellent separations and analysis via LC-MS. Alternatives to standard trifluoroacetic acid (TFA) and formic acid (FA) mobile phase conditions are shown for intact molecule separations, subunit analyses, and for analysis of tryptic digests. |
Long Abstract
The structural characterization of monoclonal antibodies (mAbs) is a challenging and complex task. Efforts have been directed at optimizing high performance HALO® BioClass Fused-Core™ silica materials in order to improve the separations component of LC-MS analysis of proteins, mAbs, glycopeptides and released glycans. Recently improved superficially porous silica packing materials have been applied to HPLC / UHPLC characterization of biomolecules including glycosylation variants from mAbs. HALO BioClass Fused-Core™ materials show considerable utility for analysis of these highly complex molecules, using conditions that permit excellent separations and analysis via LC-MS. Alternatives to standard trifluoroacetic acid (TFA) and formic acid (FA) mobile phase conditions are shown for intact molecule separations, subunit analyses, and for analysis of tryptic digests.
References & Acknowledgements:
| Description | Y/N | Source |
| Grants | no | |
| Salary | yes | MAC-MOD Analytical |
| Board Member | no | |
| Stock | no | |
| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | yes |