Barbara Bojko (Presenter)
Nicolaus Copernicus University
Authorship: Paulina Goryńska (1), Krzysztof Goryński (1), Iga Stryjak (1), Nathaly Reyes-Garces (2), German Gomez-Rios (2), Janusz Pawliszyn (2), Barbara Bojko (1)
(1) Nicolaus Copernicus University in Torun, Bydgoszcz, Poland (2) University of Waterloo, ON, Canada
| Short Abstract Analysis of clinical samples, particularly tissues, is troublesome and time consuming procedure. In the past few years progress in mass spectrometry enforced development of fast and simple methods of sample collection and introduction to MS platforms. Using brain tumor and liver and kidney graft studies as the examples of clinical applications, the presentation will demonstrate how in vivo and in situ solid phase microextraction (SPME) addresses requirements for tissue sample preparation prior targeted and untargeted LC-MS and MS analysis emphasizing features of the technology, which complement more conventional protocols. This will include phenomenon of chemical biopsy and tissue sample collection-free extraction, low invasiveness and balanced analyte coverage. It will be also discussed what areas of medicine could benefit from the proposed solution. |
Long Abstract
For the past several years mass spectrometry has been gaining a growing interest as a powerful tool enabling to perform a broad range of analysis across various areas of medicine. Combination of high resolution, mass accuracy, sensitivity and fragmentation permits for simultaneous untargeted profiling of metabolome, lipidome, and monitoring of drugs and biomarkers. The complexity and variety of biological matrices used in bioanalysis as well as specificity of different applications enforces to engage different sample preparation solutions in the utilized protocols. While preparation of biofluids is relatively straightforward procedure, preparation of tissues for (LC)-MS analysis is more demanding and not an easy task, especially considering on-site analysis. Moreover, one of the main requirements in clinical arena is fast time of overall analysis “from sample to data”. Therefore, nowadays there is a trend of simplifying the analytical workflow by omitting the most time consuming steps of “standard” analysis i.e. sample preparation and chromatographic separation. This, however, may influence quality of the data by introducing interferences present in complex biological matrices and lead to matrix suppression or enhancement.
For the past few years a utilization of solid phase microextraction in biomedical analysis has been investigated and verified. Based on the obtained data several features of the technology have been recognized to be of distinctive value in such applications, e.g. simple and uniform protocol for liquid and solid samples, efficient sample clean up eliminating or minimizing matrix effect, broad analyte coverage enabling extraction of compounds with different physical and chemical properties, analysis of tissue without its collection or consumption, flexibility of probe geometry permitting different direct couplings with MS sources. In the presentation, results of the analysis of brain tumors as well as kidney and liver transplants performed with the use of in situ and in vivo SPME coupled with nanoESI-HRMS and LC-HRMS will be shown. The results of untargeted metabolite profiling as well as targeted studies obtained with both configurations will be discussed in the view of using SPME-(LC)-HRMS solution as possible alternative that could fill the niche of fast and comprehensive tissue analysis on-site. The selected examples will include classification of brain tumor types and degree of malignancy, and changes occurred during preservation of organ grafts.
References & Acknowledgements:
[1] B. Bojko, K. Gorynski, G.A. Gomez-Rios, J.M. Knaak, T. Machuca, V. N. Spetzler, E. Cudjoe, M. Hsin, M. Cypel, M. Selzner, M. Liu, S. Keshjavee, J. Pawliszyn. Anal. Chim. Acta, 803, 75 (2013)
[2] B. Bojko, K. Gorynski, G.A. Gomez-Rios, J.M. Knaak, T. Machuca, E. Cudjoe, V. N. Spetzler, M. Hsin, M. Cypel, M. Selzner, M. Liu, S. Keshjavee, J. Pawliszyn, Lab. Invest., 94, 586 (2014)
[3] E. Cudjoe, B. Bojko, I. Delannoy, V. Saldivia, J. Pawliszyn. Angew. Chem. Int. Ed., 52, 12124 (2013).
Acknowledgments:
1. National Science Centre, Poland, grant 2015/18/M/ST4/00059
2. Thermo Finnigan LLC
3. Supelco Inc., part of MilliporeSigma
| Description | Y/N | Source |
| Grants | yes | National Science Centre, Poland, grant 2015/18/M/ST4/00059 |
| Salary | no | |
| Board Member | no | |
| Stock | no | |
| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | no |