MSACL 2023 Abstract

Online SPE-HPLC-MS/MS Method for Measuring Metabolites of UV Filters in Human Urine

Lei Meng, Xiaoliu Zhou, Kyle Smith, Antonia Calafat, Julianne Cook Botelho
Centers for Disease Control and Prevention

Lei Meng (Presenter) Centers for Disease Control and Prevention

Relevant Financial Disclosures (within past 24 months) No relevant financial relationship(s) to disclose. Conflict mitigation NOT REQUIRED.

INTRODUCTION: Sunscreens are widely used as over-the-counter products in the United States. Avobenzone, oxybenzone, octocrylene and 2-ethylhexyl salicylate are active ingredients commonly used in sunscreen formulations and other personal care products. Previously many of these active ingredients were detected in plasma, amniotic fluid, breast milk and urine. However, little is known about safety of these active ingredients. Due to their widespread use, exposure data can inform evaluations on the safety of these chemicals.

METHODS: We have developed a method using enzyme deconjugation, isotope dilution, on-line solid phase extraction (SPE) coupled with high performance liquid chromatography-isotope dilution tandem mass spectrometry (HPLC-MS/MS) to measure the metabolites of avobenzone, oxybenzone, octocrylene and 2-ethylhexyl salicylate in human urine, namely, di-hydroxy avobenzone (DHAVO), 2-ethyl-5-hydroxyhexyl 2-cyano-3,3-diphenyl acrylate (5OH-OC), 2-ethyl-5-hyroxyhexyl 2-hydroxybenozate (5OH-EHS), 2-ethyl-5-oxohexyl 2-hydroxybenozate (5oxo-EHS), and 5-(((2-hydroxybezoyl)oxy)methyl) heptanoic acid (5cx-EPS).

RESULTS: In this method, we use 100uL of urine. The limits of detection range from 0.02 to 0.1 ng/mL. The intra-day precisions range from 2.8-6.3%, inter-day precisions range from 5.4-10.6%. The accuracy using spike recoveries range from 86%-124%. Detection frequency in a convenience sampling from anonymous volunteers (N=110) range from 14.5-44.5%. This method is sensitive and rugged. In addition, with this method we can measure other phenolic compounds such as bisphenol A, bisphenol F, bisphenol S, benzophnone-3, triclosan, triclocarban and parabens.

CONCLUSION: We developed a sensitive and robust method to measure metabolites of four UV filters in sunscreen products in human urine samples. This method is suitable for large epidemiological studies and for biomonitoring studies to estimate the prevalence of human exposure to these compounds.