MSACL 2024 Abstract

Self-Classified Topic Area(s): Practical Training > Various OTHER

Podium Presentation in Colton on Thursday at 13:30 (Chair: Deborah French)

Clinically available. Everything you wanted to know about Internal Standards, but were too afraid to ask
Russ Grant
Labcorp, Burlington, NC

Russell Grant, PhD (Presenter)
Labcorp

Presenter Bio: Dr. Grant earned a first-class honors degree in Industrial Chemistry from Cardiff University and a PhD in Chromatographic and Mass Spectrometric technologies from the University of Swansea, Wales, United Kingdom. He continued his scientific training in various industrial settings, which have included senior scientist at GSK, Principal scientist at Cohesive Technologies, Technical director at Eli Lilly, and Director of Mass Spectrometry at Esoterix Endocrinology. Dr Grant is currently the Vice President of Research and Development and co-discipline director for Mass spectrometry at Labcorp. Dr Grant has pioneered the use of direct injection technologies, chromatographic systems multiplexing, microsampling, utility of automation, and other new analytical platforms in direct patient care. His research goals are focused upon improvements in speed, sensitivity, and quality of liquid chromatography with tandem mass spectrometric (LC-MS/MS) analytical systems and assays. Dr Grant has been awarded 100 patents and received both the MSACL Distinguished contribution award and ASMS AL Yergey “Unsung Hero” Award in 2024 for his contributions to Clinical Diagnostics using Mass Spectrometry.

Abstract

Structure: This one hour teaching session regarding Internal Standards will be delivered in three 20 minute vignettes. The first session will describe in detail the "What, Why and How" Internal standards should be used in isotope dilution LC-MS/MS assays. The second session "But what about when?" will describe situations where Internal Standards fail criterion for use and how to correct deficiencies for effective LC-MS/MS assays. The third session will describe the "Unique capabilities" afforded to analytical measurement when used as pre-analytic correction tools, internal calibrators, discrimination between recovery and ionization effects (duplicate IS's) and in method development (as surrogates for analytes).

Take Home Pearls: Key experimental considerations and "cause-effect" will be detailed to establish when internal standards are behaving in a manner consistent with the analyte.

Session 1 will include: What level should I add IS at? what does the IS actually do? Qualitative details and error observations, quantitative assessment of binding equivalency (Reverse admixing - and when it can be misleading), drift over time using dynamic extraction and automation tools (and how to both elucidate and correct errors).

Session 2 will highlight examples of internal standardization failures such as the impact of excessive labeling, Isotopic contribution (from and to analyte), and when to use an "analog" internal standard - correctly.

Session 3 will detail expanded uses of Internal standards such as differentiation between recovery error and ionization effects (to confidently determine release or repeat for patient results), in-plate dilution for results reporting outside the calibration range and how an internal standard can be used as a calibration system (and when not to!).

Financial Disclosure

Description	Y/N	Source
Grants	no
Salary	yes	Labcorp
Board Member	no
Stock	yes	Labcorp
Expenses	yes	MSACL
IP Royalty	no

Planning to mention or discuss specific products or technology of the company(ies) listed above:
no