MSACL 2024 Industry Workshop Presentation *This Podium Presentation is occuring in the context of an Industry Workshop that starts at the time below. Its actual start time may be up to 40 minutes later, depending on order of presentation if there are multiple presentations planned by the workshop host. Workshop Host: Thermo Fisher Scientific Day: Wednesday March 20 Time: 7:30* Location: Steinbeck 1 (Conference Ctr > 2nd Floor)

An optimized, simplified workflow for the targeted proteomic analysis of fibrinogen in 4,200 plasma samples
	Stefani Thomas, PhD, DABCC, NRCC University of Minnesota
Presenter Bio: Dr. Stefani Thomas is an Assistant Professor in the Department of Laboratory Medicine and Pathology at the University of Minnesota, and the Associate Medical Director of the M Health Fairview University of Minnesota Medical Center West Bank Laboratory. She earned a BA in Biological Sciences from Dartmouth College, a PhD in Pharmaceutical Sciences from the University of Southern California, and she completed a Clinical Chemistry postdoctoral fellowship at Johns Hopkins. Her research program at the University of Minnesota utilizes mass spectrometry-based clinical proteomics for therapeutic and diagnostic biomarker development.

The plasma concentration of fibrinogen – the major structural component of blood clots – is a critical indicator of hemostasis. Although plasma fibrinogen immunoassays performed in clinical laboratories generally have high accuracy and sensitivity, they require a large sample volume and they are relatively expensive. To avoid these limitations for the analysis of plasma fibrinogen in a longitudinal clinical research study (n=4,200 participants), we developed and validated a targeted mass spectrometry assay using a Thermo Scientific™ Q Exactive™ Plus mass spectrometer coupled with a Thermo Scientific™ Vanquish™ UHPLC system, and we streamlined the sample processing workflow to successfully achieve a throughput of ~90 samples/24 hours.