MSACL 2023 Industry Workshop Presentation *This Podium Presentation is occuring in the context of an Industry Workshop that starts at the time below. Its actual start time may be up to 40 minutes later, depending on order of presentation if there are multiple presentations planned by the workshop host. Workshop Host: Agilent Technologies Day: Thursday April 6 Time: 7:00* Location: Steinbeck 2

Ultra-high sensitivity for targeted proteomics using Evosep-MRM at the single cell level
	Angela Mc Ardle, PhD, MSc, BSc Evosep Biosystems
Presenter Bio: Angie is a Senior Scientist at Evosep Biosystems where her focus is proteomics and clinical applications. She has a strong background in mass spectrometry-based proteomics and has gained experience in multi-omic biomarker studies integrating both discovery and targeted approaches. Angie is originally from Ireland and received her PhD at University College Dublin. She continued her research career at Cedars Sinai, Los Angeles before joining Evosep Biosystems based in Denmark.

The Evosep One is a powerful and standardized platform for high sensitivity work including single cell proteomics. The robustness and throughput of the Whisper methods on the Evosep One combined with the sensitivity of the Agilent 6495C triple quadrupole support targeted analysis of proteomes at the single cell level. We demonstrate this by targeting selected peptides in a complex background of sub nanogram material combined with dilution curves spanning several orders of magnitude. To ensure seamless integration of the EvosepOne with the Agilent 6495C triple quadrupole, we have developed a native driver for MassHunter and a robust solution for the Agilent nanoflow ion source.