PRE-REGISTER for this Workshop
| Day: | Wednesday |
| Time: | 16:00 - 16:30 |
| Room: | Mozart 4-5 |
Quantitation of endocrine markers using High Resolution Mass Spectrometry
Laura Owen – University Hospital of South Manchester
Traditionally the quantitation of endocrine biomarkers has been performed using triple quadrupole analysers whereas orbitrap technology lends itself primarily to drug monitoring and screening applications. However, in recent years, orbitrap high resolution mass spectrometry systems have proved to be a great screening and quantitation tools.
Our research goal was to quantify renin activity, aldosterone and metanephrines using orbitrap HRMS, in order to bring an added level of capability and flexibility to our lab. For plasma renin analysis, data was acquired in both PRM and full scan modes while, for aldosterone analysis, extract was analysed by PRM only with negative ion mode. Renin activity was measured at concentration level typically found in Conn’s syndrome with sufficient sensitivity. PRM showed slightly superior sensitivity than full scan mode. Metanephrine and normetanephrine both gave adequate sensitivity and calibration lines however 3-methoxytyramine was not sufficiently sensitive. Aldosterone demonstrated good sensitivity in sub 100pmol/L concentrations.
The QE focus demonstrated its ability to quantify difficult to measure endocrine markers offering quantitation capabilities along with full scan thus bringing screening, protein discovery and quantitative capabilities to the clinical laboratory.