|08:00 - 08:45
Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) are unique biomarkers of alcohol use. EtG and EtS analysis offers many advantages for abstinence monitoring including the detection window, stability in stored specimens, and specificity. EtG and EtS are both polar, making them difficult to retain via reversed-phase chromatography. Both compounds are also very sensitive to matrix interferences which can result in being unable to achieve low limits of detection and can also make quantitation impossible. In this study, a simple dilute and shoot method was developed and validated for the analysis of EtG and EtS in human urine by LC-MS/MS.