|12:30 - 13:15
Innovative Filtration Method to Rapidly Remove Protein from Biological Matrices Prior to LC/MS/MS Analyses
Daniel B. Kassel, Ph.D., Founder & CEO, SciAnalytical Strategies, Inc., La Jolla, CA; Kaylee R. Mastrianni, Ph.D., Applications Chemist, DPX Technologies, LLC,
Conventional protein precipitation methods require centrifugation and oftentimes offline sample handling prior to LC/MS/MS analyses. We introduce a rapid and readily automated patent pending method for protein precipitation with automated filtration. The method utilizes Tip-On-Tip (ToT) technology for the streamlined analysis of urine, whole blood, and serum/plasma. We will discuss numerous applications such as filtration of hydrolyzed urine samples, comprehensive drug testing in whole blood, and steroid analysis in serum. We will also present a validated method for the analysis of total testosterone in serum, and show detailed patient results obtained from a collaborative clinical laboratory. Using this ToT technology, up to 96 serum samples were processed simultaneously in less than 10 minutes using a Hamilton Nimbus96, providing clean extracts immediately ready for injection into LC/MS instrumentation.