|07:00 - 07:45
Sample Preparation for Meaningful LC-MS
Mass spectrometry-based analysis is playing an increasingly vital role in basic research and more significantly, clinical applications. Sample preparation is an integral, but often overlooked, component of a successful LC-MS experiment. However, given the highly manual nature of the process, dearth of efficient standardized methods, and the wide variability in sample content, sample processing is the predominant source of errors and reporting bias. Due to the nature of the workflow, sample prep errors remain undetected until the final data analysis step; such issues are particularly costly in clinical settings where samples are precious or access is limited.
This workshop will offer simple tips and tricks to expedite sample preparation while ensuring sample integrity is maintained. Aspects of sample preparation to be covered in the workshop include:
- Quick guidance to filtration membrane selection allowing efficient and contamination (extractables) free sample cleanup
- Importance of plate selection for high throughput removal of matrix components while retaining desired analyte(s)
- Filter Aided Sample Preparation (FASP) and attempts on quantitative analysis
- Quick ZipTip based sample pre-treatment to enhance LC-MS quality