Corporate Workshops

Wednesday
8:00 - 8:45

Wednesday and Thursday
14:00 - 14:45

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Smart Solutions for Clinical Applications
Dr. Antonio D'AVOLIO, Neil LOFTUS

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The Internal Standard Normalized Matrix Effects (IS-nME): a new tool for the mass spectrometry method validation and its use in clinical applications
Dr. Antonio D'AVOLIO - Università degli Studi di Torino / ASL TO2 - Turin (Italy)

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Quantitative Multi Target Screening (MTS) using liquid chromatography-tandem mass spectrometry with MS/MS library based identification for clinical toxicology.
Neil LOFTUS - SHIMADZU MS Business Unit Overseas - Manchester (UK)

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For Research Use Only. Not for use in diagnostic procedures.

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Speeding up the cancer biomarker discovery: Advanced Clinical Proteomics workflow with High Resolution MS
Sebastien Gallien- Former researcher at CRP-Santé LIH Luxembourg Insitute of health

Adoption of cancer biomarkers in clinical labs has been plagued by the speed with which these biomarkers are discovered and validated for clinical use. Clinical proteomics approaches where the use of predefined set of surrogate peptides for proteins has been helpful in systematically identifying these markers. Targeted analyses using parallel reaction monitoring (PRM), performed on high resolution and accurate-mass (HRAM) quadrupole-orbitrap mass spectrometers, present the selectivity and sensitivity to confidently quantify peptides in complex samples. The internal standards (IS) used for isotope dilution quantification were recently leveraged to actually drive the acquisition (“internal standard triggered-parallel reaction monitoring”, IS-PRM), thus improving the acquisition efficiency.

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Metabolomics in Clinical Research
Dr. Andrew Davison, Dr. Christopher Gerner

Metabolomics of Fingersweat: Individual Assessment of Metabolic Parameters based on Fingerprint Analyses
Christopher Gerner, Institute of Analytical Chemistry, University of Vienna

Non-Targeted Metabolomic Evaluation of patients with Alkaptonuria (AKU)
Andrew Davison, Department of Clinical Biochemistry and Metabolic Medicine, Liverpool Clinical Laboratories, Royal Liverpool & Broadgreen University Hospital Trust, UK

For Research Use Only. Not for use in diagnostic procedures.

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The Microsampling Workshop
Stephen Stephenson, Chief Biomedical Scientist of Transplant, Cellular, and Clinical Immunology, Leeds Teaching Hospital; Dr. Bikalpa Neupane, Research Fellow University of Leicester; John Dutton, Senior Research Fellow and Holly Nicholls, Research Technician, University of East Anglia Bioanalytical Facility

This workshop examines three clinical research labs investigating volumetric absorptive microsampling (VAMS™) as an alternative to “wet” sampling. The first talk reviews a potential at-home LC-MS/MS assay for tacrolimus monitoring including extractions and initial validation results. Talk two describes a clinical PK children’s study which aims to compare PK of midazolam in critically-ill with that in otherwise healthy children undergoing elective surgery and measure blood midazolam concentrations in “wet” sample against those in VAMS. The final talk discusses a LC-MS/MS assay for the quantitation of 25(OH)D3 in whole blood collected using DBS and VAMS. The two microsampling techniques are compared against plasma and the impact of different haematocrit levels on the 25(OH)D3 concentration is investigated.

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Microsampling Techniques and LC-MS/MS: Therapeutic Drug Monitoring Research to Help Personalize Medicine for Our Children
Giuliana Cangemi, PhD, MSC - Istituto Giannina Gaslini Children

The first part of this session will focus on some basic principles of TDM in pediatrics. Analytical challenges and opportunities will be explored. The second part of this session will focus on alternative sampling strategies such as dried blood spot, volumetric microsampling devices and dried plasma spot coupled to tandem mass spectrometry. Application to routine clinical analysis in pediatrics will be discussed.

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New MS Aspects for Biology Driven Metabolomics and Proteomics in Translational Research
Prof. Dr. Thomas Hankemeier Leiden University, Netherlands & Dr. Jörg Dojahn, SCIEX, Germany

During this seminar two talks will be presented by Prof. Dr. Thomas Hankemeier Leiden University, Netherlands & Dr. Jörg Dojahn, SCIEX, Germany The first talk will cover MS-based Platforms for Targeted and Biology-driven Metabolomics, whereas the second one will focus on high throughput SWATH® acquisition for proteomics in translational research.

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New Technologies in Affinity Mass Spectrometry and Colony Picking
Petr Pompach, Jaroslav Hrabak, Helena Husakova

BioVendor is a consistent leader in the development of top quality immunodiagnostics and biomedical research tools. The workshop is divided into two sessions: In the first session, the antibody-functionalized MALDI surfaces prepared by ambient ion landing will be described. The effectivity of antibody-functionalized MALDI surfaces to enrich protein antigen form serum will be demonstrated on determination of haptoglobin phenotype - an important biomarker in patient survival. The second session will be focused on presentation of the intelligent robot for colony picking and complete MALDI target preparation – MALDI COLONYST®. The robot improves MALDI identification scores up to 15% by utilizing principle of patented adaptive geometry and wet deposition. All steps of process are recorded, which makes system fully transparent and documented.

Latest Advancements in High Content Protein Quantification
Karel Novy, Fadi Abdi

Biognosys is the leading proteomics company offering services and tools for highly multiplexed protein quantification. Its cutting-edge technology has been applied in biomarker research, drug and target discovery, pathway modeling, mode of action studies, and many other areas. Two Biognosys’ platforms for protein analysis will be presented in the workshop: discovery and targeted proteomics. Discovery proteomics platform allows an unmatched proteome coverage with accurate quantification of up to 8’000 proteins per sample, and is ideal for identifying over-expressed proteins on a proteome level without the need for any previous knowledge. On the other hand, the targeted proteomics platform allows an absolute quantification of up to a 150 pre-defined proteins that can be analyzed in a high-through put mode in thousands of samples.